High Performance Liquid Chromatography

H igh Performance Liquid Chromatography (HPLC) is an essential analytical tool in any testing lab as it affords separation and quantification of a variety of analytes which are thermally labile and not volatile enough for analysis on gas chromatograph.
We have 2 HPLC instruments from Jasco and Agilent G7111B with cobra cell equipped with a variety of detectors. The number of instruments is indicative of the utility of the technique in the laboratory.
The different modes and choice of detectors permit analysis of wide range of samples, and we have the following detectors available in our testing laboratories –
• Diode Array Detector
• Refractive index (RI) detector
• Photo diode array detector (PDA)
• Fluorescence detector
The HPLCs in our testing lab are equipped with auto-samplers AS-4050 for 24×7 operation and higher accuracy and precision. This enables us to provide reliable results and lowest turnaround time (TAT).

The large variety of stationary phases available make it one of the most versatile techniques and it can be used for the analysis of molecules like :
• Small molecules
• Semi polar molecules by reverse phase chromatography
• Macro-molecules by Gel permeation chromatography
• Polar molecules in Cyano or Amino colums and Ion pairing chromatography
• Nonpolar molecules using Silica of C1-C4 & C18 colums

HPLC is used in our testing to analyze products from the following industries –
• Pharmaceuticals
• Foods
• Fine chemicals
• Colors and dyes
• Bio molecules and
• Environmental analysis
• and many more

We routinely carry out the following analysis on HPLC –
• Assay of drugs products and formulations
• Impurity profiling of pharmaceuticals
• Quantification of Vitamins
• Estimation of caffeine
• Estimation of sugar
• Estimation of Aflatoxins, Ochratoxin
• Estimation of Antioxidant
• Estimation of coloring agent
• Estimation of Antibiotic (Histamine)
• Method development
• Method validation